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R & D

Past achievements and complete research projects

Extraction and purification of IAA from P. florescence

Indole acetic acid (IAA) production is a major property of rhizosphere bacteria that stimulate and facilitate plant growth. The present work deals with identification of Indole Acetic Acid producing bacteria. Five bacterial culture (Bacillus subtilis, Bacillus megaterium. Pseudomonas polymixa, Azotobacter and Pseudomonas flourenscence) were used for the test. Among five, Pf was selected as efficient producers. Optimization of indole acetic acid production was carried out at different tryptophan concentration. It was found that Pf produced highest IAA at 0.02mg/ml concentration of Tryptophan after 48 hrs of incubation and gradually decline after 72 hrs (Fig.1).

Study of mycorrhiza on maize plant.

ARBUSCULAR mycorrhizal (AM) fungi are obligate symbionts and are grown in association with living tissues. Several culture techniques based on this constraint are applicable for commercial scale production of the inoculums. The most widely used is pot culture, where the fungi are usually maintained and multiplied in conjunction with suitable host plant roots. In this process isolated vam from maize soil were purified and treated with maize seedling and left for plant growth. After 30 days roots were harvested, the observed mass of root was 0.82g and height was 14.5 cm as compared to control (Fig 2 and 3).

Study of Biohit product of Trichoderma spp. on potato farming

Trichoderma viride proved as one of the most important fungus for farming. Along with the properties of pesticides it also acts as biofertilizer by releasing phosphorus and other PGPR and making it available for plants to grow. A trial plot as developed to check the efficiency of IB product Biohit. Seed treatment was given to potato seeds as per prescription and sown at Rangwasa Jaivik Gram field. After one month 100% seed germination was observed without any disease (Fig. 4). After germination one more treatment of Biohit was given to the plants. After two months the crop withstand with good quality (Fig.5)

Extraction of Siderophores from Psudomonas Fluorescence

Siderophores, a high-affinity chelating agents for ferric iron, is one of the mechanisms by which biocontrol agents' act in inhibiting the growth of phytopathogens. Pseudomonas flourences, a siderophore producer, is a potential biocontrol agent. In this study, a simple double-layered chrome azurol S agar (SD-CASA) plate assay was developed. After incubating culture we found out that the LB medium presented strong significant CAS reaction even for 12 h, and with the help of SD-CASA plate assisting paper-disc diffusion, strain Pseudomonas fluroscence from LB medium with Fe3+ (10 μmol/L) showed difference interestingly. In addition, CAS reaction and growth reaction also increased with pH 7.0, and carbon-nitrogen ratio 5:1. The SD-CASA assay is a useful tool for screening potential biocontrol microorganisms by siderophore production.)

Standardization of solid state culture of Trichoderma spp. In open tray and study of shelf life in production and packing.

Trichoderma is one of the most important fungus used in agriculture as biopesticide and PGPR. Our attempt was to minimize the growth cycle from 16 days to 5-6 days and obtain maximum sporulation. When Trichoderma spps were grown on different grains like corn, wheat grains, gram, jowar and bajara it was found that bajara showed good result as compaired to others.

Ongoing Projects
Isolation, purification and biochemical analysis of Phosphorus Solubilizing Bacteria from different soil samples of different parts of India.
Isolation, purification and biochemical analysis of Potash Solubilizing Bacteria from different soil samples of different parts of India.
Isolation, purification and biochemical analysis of Zinc Solubilizing Bacteria from different soil samples of different parts of India.
Isolation, purification and biochemical analysis of Azotobacter, Azospirullum from vermicompost and different soil samples of different parts of India.
Standardization of mass scale culture of Trichoderma spps. from poly bags to culture tray.

Future Projects
To develop genetically modified probiotic bacteria against harmful fungus in Agriculture.
To scale-up production of IAA on large scale from lab to production unit.
Screening and purification of bacteria and fungus producing PGPR.
To develop methods for preserving and increasing shelf life of harvested spores of fungus.
To develop methods for quality check of strain performance.
Method to check product performance in trial plots.

Research publications
Anukriti Verma, Yamini Patidar, Aditi Vaishampayan (2016), Isolation and purification of potassium solubilizing bacteria from different regions of India and its effect on crop's yield. Indian Journal of Microbiology Research: 3(4),482-487.
Deepak Kewat, Anis Siddiqui, R.K Jain (2016), Isolation and preliminary Characterization of effective and complete strain of Rhizobium from non-traditional legume crops of Indore (M.P). Indian Research Communication:10(1), 50-59.
P.K Singh, R.K. Jain, Aditi Vaishmpayan, Sapna Parihar (2014), Biocontrol of Rhizoctonia sheath blight disease of Rice using Biohit (Trichoderma viride) WP formulation. Indian J. Plant Pathology, 31.
P.K Singh, R.K. Jain, Aditi Vaishmpayan, Sapna Parihar (2014), Management of Damping off (Phytium aphanidermatum) in chilli (Capsicum annum cv VNS-4) by Pseudomonas flourescences. International Journal of Agriculture, Environment and Biotechnology, 7(1):83-86.

Abstracts
R.K Jain, A. Shrivastav, D.K. Sharma, Bioeffective selection to improve virulence potential of entomopathogenic fungi strain. National Seminar on Application of biotechnology to improve industrially important microbial strain, March 2012.
H. Bhojne, R.K Jain, A. Shrivastav, D.K. Sharma, Optimization of pH to improve semisolid fermentation of Btk. National Seminar on Application of biotechnology to improve industrially important microbial strain, March 2012.
Pooja Upasani, R.K Jain, H. Singh, Parmar, R. Sharma, S. Tyagi, Isolation of Plant Gowth Promoting and Biocontrolling effective strains of Pseudomonas fluroscence. National Seminar on Application of biotechnology to improve industrially important microbial strain, March 2012.
H. Bhojne, R.K Jain, R. Sharma, Isolation of complete and effective Azotobacter strains from different Rhizospheric soil. National Seminar on Application of biotechnology to improve industrially important microbial strain, March 2012.

Detail of scientific persons working in R and D unit and their qualifications


# Name Qualification Designation
1 Dr. Jayant Bhole Ph.D Microbiology
2 Dr. Anukriti Verma Ph.D Biotechnology
3 Ms. Adity vaishampayan MSc. Biotechnology
4 Sushil kumar 12th labour

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